Biochemical study of DNA markers for Bacterial infection in bovine mastitis

Abdel-maged, Afaf D.; El Sheita, Wael A.; Abdelwahab, Mohamed G.

doi:10.21608/bvmj.2016.31275

Biochemical study of DNA markers for Bacterial infection in bovine mastitis

Document Type : Original Article

Authors

¹ Faculty of Veterinary Medicine, Benha University

² Biochemistry department, Faculty of Veterinary Medicine, Benha University

³ Animal medicine department, Faculty of Veterinary Medicine, Benha University

10.21608/bvmj.2016.31275

Abstract

Mastitis is a multifactorial disease and very difficult to control. It results from injury, chemical irritation and infection
caused by different bacterial species. Mastitis remains one of the most common economic problems of dairy industry
worldwide as it is the most expensive disease of dairy animals resulting in the reduction of milk production and quality.
These expenses in terms of reduction of production, discarding milk, drug therapy, veterinarian charges, culling of
incurable animals and extrause of labor. Analysis of bacteriological examination of milk samples was made to identify
the main etiological agents involved in the disease. The organisms were identified on the basis of their cultural, staining
characteristics, biochemical reactions .and molecular detection. Milk sample of 23 cows, which were positive for
California mastitis test, cultured for microbiological examination in the study period. Two bacterial species were isolated,
Staphylococcus aureus (Staph. aureus) and E. coli bacterial isolates. The predominant isolated bacteria were Staph. aureus
with isolation rate of 37.77% however E. coli was isolated with isolation rate of 13.33%). Serum alkaline phosphatase
(ALP) enzyme and calcium levels were highly significant decreased while C-Reactive protein (CRP) titre and phosphorus
levels were highly significant increased. Lactate dehydrogenase enzyme(LDH), Aspartate aminotransferase
enzyme(AST), Gamma-glutamyl transferase enzyme(GGT), Albumin, sodium, potassium and chloride levels were nonsignificantly
changed in serum of mastitic cows compared to healthy ones. While LDH, ALP and phosphorus levels were
highly significant increase in milk of mastitic cows compared to that of healthy ones. However, the calcium level was
high significantly decreased in mastitic cows compared to healthy ones. Molecular detection of Staph. aureus and E. coli
isolates by PCR. The expected sizes of PCR products of Staph. aureus was (984bp), while that for E. coli were (662bp).

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