Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ.

2 Microbiology Dept., Fac. Vet. Med. Zagazig Univ.

3 Animal Health Research Institute Dokki, Giza

4 General Authority for Vet. Services

Abstract

Two hundred random samples of milk, kareish cheese, yoghurt and ice-cream (50 for each) were examined
microbiologically for the presence of Clostridium perfringens, their enterotoxigencity and their antibiotic sensitivity.
Clostridium perfringens was isolated from 3 (6%) milk samples, 4 (8%) kareish cheese samples and it could not be
isolated from any examined samples of yoghurt and ice-cream. The majority of C. perfringens isolates recovered from
milk and milk products were susceptible to ofloxacin, ampicillin + sulbactam and norfloxacin (100%), vancomycin,
tetracycline, metronidazole and amoxicillin + clavulinic acid (83.3%) and clindamycin (66.7%). The majority were
resistant to cephalothin (100%), sulphamethoxazole + trimethoprim (83.3%), oxacillin and chloramphenicol (66.7%).
Molecular studies using multiplex PCR technique for detection of alpha toxin gene and C.perfringens types "A"
enterotoxin gene revealed that the 7 isolates of C. perfringens (100%) were positive for alpha toxin gene and only 2 out
of 7 isolates (28.57%) were positive for enterotoxin gene

Keywords

Benha Veterinary Medical Journal
Volume 31, Issue 2
December 2016
Pages 144-148

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