The hepato- protective Effect of Stem Cells and Levamisole against Carbontetrachloride induced Liver Fibrosis

Document Type : Original Article

Authors

1 Clinical Pathology department, Faculty of Veterinary Medicine, Benha University

2 Pathology department, Animal Health Research Institute

Abstract

This study designed to evaluate the hepatoprotective effect of stem cells only or stem cells and levamisole on
experimentally Carbon tetrachloride (CCl4) induced liver damage by evaluation of haematological parameters,
biochemical parameters, immunological parameters and histopathological findings. Sixty (60) Wister rats were
divided into four groups with 15 animals in each group. Group (1): normal group. Group (2): rats injected with
CCL4 (0.2ml/100gm) s/c twice a week for 9 weeks. Group (3): rats injected with CCL4 then given MSCs
(3x106 cell) I/v once a week for 4 weeks. Group (4): rats injected with CCL4 then given levamisole (2.5mg/kg)
three successive days for 4 weeks and stem cells (MSCs) (3x106 cell) I/v once a week for 4 weeks. Results
showed that injection of CCL4 lead to significant increase in activity of hepatic enzymes (AST, ALT, ALP)
and Bilirubin level while it decreased serum total protein, albumin, IL- 6, Phagocytic index, Phagocytic %,
IgG and IgM. While after treatment with stem cells only or stem cells and levamisole lead to significant
decrease in activity of hepatic enzymes (AST, ALT, ALP) and Bilirubin level while it increased serum total
protein, albumin, IL- 6, Phagocytic index, Phagocytic %, IgG and IgM

Keywords

Benha Veterinary Medical Journal
Volume 31, Issue 2
December 2016
Pages 149-157

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