Molecular characterization of Aminoglycosoide and Tetracycline resistant Salmonella isolates causing new born ruminants diarrhea

Document Type : Original Article

Authors

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University

Abstract

Ten isolates of Salmonella species were obtained from sporadic cases of profuse diarrhea in new
born ruminants from El-Menofiya and El-Kalubia Governorates in Egypt. These isolates were as follow
7 isolates from calves (Salmonella Typhimurium, Salmonella Enteritidis,two were Salmonella
Saintpaul, two were Salmonella Langeveld and Salmonella Havana) ,two isolates from lambs
(Salmonella Typhimurium, Salmonella Bardo) and one isolate from goat-kids(Salmonella
Enteritidis). Salmonella isolates resistance to aminoglycosoides was 0.00% for Amikacin, 30% for
tobramycin and 50% for streptomycin. Susceptibility of isolates to tetracycline was 50% for
doxycycline. Results showed a high incidence of aminoglycosoides resistance gene aadB in 100% of
the isolates while aadA2 genes in 40% of the isolates. Incidence of tetracycline resistance genes was
0.00% for tetA(B) and 80% for tetA(A). The difference between the results of this study and those from
other regions in Egypt necessitate a complete survey overall the country to make a complete and clear
map of salmonella servoars, their antibiotics susceptibility and in molecular characterization of
resistance determinants in each region

Keywords

Benha Veterinary Medical Journal
Volume 30, Issue 1
March 2016
Pages 161-170

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