Molecular characterization for some virulence and antibiotic resistance genes of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL- Sharkia Governorate

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.

2 Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Zagazig Univ.

3 Animal Health Research Institute (Zagazig branch)

Abstract

Staphylococcus aureus has emerged as a significant public health problem as it is often responsible for
intramammary infection in bovine. The emergence of MRSA in animals was from an outbreak of
mastitis in cattle which represent a great economic in milk industry. The present study was carried
out to genotypically characterized S. aureus isolated from subclinical bovine mastitis in different farms
in EL- Sharkia Governorate. A total of 15 S. aureus isolates were obtained from 100 subclinical mastitic
milk samples and subjected to PCR for detection of some virulence and antimicrobial resistance genes
using oligonucleotide primers that amplified genes encoding enterotoxin genes A to E (sea, seb, sec,
sed, see), coagulase gene (coa), the IgG binding region of protein A (spa) and resistance gene as factor
essential for expression of methicillin resistance (femA) which was used as an internal positive control
and intrinsic methicillin resistance gene (mecA). PCR amplification revealed that all S. aureus isolates
were enterotoxogenic and MRSA, harbored the genes encoding staphylococcal coagulase and the genes
encoding the immunoglobulin G binding region of protein A. The data in the study provided an overview
on the distribution of virulence determinants of MRSA strains which contributed to bovine mastitis
problem in the Egypt farm.

Keywords

Benha Veterinary Medical Journal
Volume 30, Issue 1
March 2016
Pages 219-230

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