Biochemical effects of ear infections by Pseudomonas aeruginosa on rabbits

Mahfouz, Mohamed K.; Youseif, Mahmoud M.; El-Mashad, Abdel-Baset I.; Shendy, Mohamed

doi:10.21608/bvmj.2016.31371

Biochemical effects of ear infections by Pseudomonas aeruginosa on rabbits

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Benha University

² Department of Pathology, Faculty of Veterinary Medicine, Benha University

³ Department of ENT, Faculty of Medicine, Benha University

10.21608/bvmj.2016.31371

Abstract

Ear infections (particularly, otitis media) is a prevailing and common infection in developing countries
causing local damage and threatening complications. Pseudomonas aeruginosa is the most common
pathogen causing chronic suppurative otitis media (CSOM) and malignant otitis externa. The objective
of this study is to identify incidence of Pseudomonas aeruginosa involved in ear infections and
associated biochemical parameters which may be changed. External auditory canal in rabbit ears was
inoculated with (10⁶) colony-forming units (CFU) of Pseudomonas aeruginosa or left as sterile controls
for eight weeks. There are significantly decrease results at (P<0.05) of Interleukin-2 and significant
increase results of Interleukin-9 and Tumor Necrosis Factor-alpha (TNF-α) in acute or chronic
infections. Moreover, histopathological changes of ear, kidney and liver tissues were also observed.
Evaluation of these parameters were needed to use of specific blocking agents to inflammatory
mediators with the aim of discovering new treatment options for chronic otitis media, and to help the
physicians in diagnosis these diseases in elderly stages and try to reduce the pain and suffering associated
with otitis media.

Keywords