Glutathione peroxidase-1 and klotho gene expression in acute renal failure rats and its association with creatinine and urea levels

Document Type : Original Article

Authors

1 Animal Wealth Development Dept., Fac. of Vet. Med., Benha Univ.

2 Animal Wealth Development Dept., Fac. of Vet. Med., Alexandria Univ.

3 Theriogenology Dept., Fac. of Vet. Med., Benha Univ.

4 Biochemistry & Mol. Biology. Fac. of Medicine- Benha Univ.

Abstract

The main objectives of this work was to study the effect of glycerol induced acute renal injury on
Glutathione peroxidase (GPx-1) and klotho gene expression which used as early indicator for acute renal
failure. Twelve male albino rats divided into two groups were included in this study. Blood samples
were taken for assessment of creatinine and blood urea nitrogen. Kidney tissue specimens were
processed for mRNA gene expression study of Glutathione peroxidase (GPx-1) and klotho genes by real
time polymerase chain reaction (RT-PCR). The creatinine and blood urea nitrogen significantly
increased (4.97± 0.75 and 242.03 ± 41.93) compared with control group (0.67 ± 0 .06 and 24.43 ± 2.18).
Moreover, the renal Glutathione peroxidase-1 and klotho gene expression was found to be decreased in
the glycerol treated group (affected group), to 0.899 and 0.91fold. Also a correlation between gene
expression of Glutathione peroxidase-1and klotho gene with the level of creatinine and blood urea
nitrogen was detected.

Keywords

Benha Veterinary Medical Journal
Volume 29, Issue 1
September 2015
Pages 97-104

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