Assessment of two stabilizers used for lyophilized live attenuated peste des petits ruminants (ppr) vaccine

Document Type : Original Article

Authors

1 Department of virology, Faculty of Veterinary Medicine, Benha University

2 Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo

Abstract

The effect of using Weybridge medium (WBM) and polyvinyl pyrrolidone-NZamine (PPNZ) as
Stabilizers during freeze-drying process and under varying storage temperature conditions on peste des
petits ruminants (PPR) vaccine was investigated. It was found that both stabilizers gave good freeze
dried preparation of PPR virus Nigeria75/1vaccines. During the process of freeze-drying, there was a
total decrease of 0.6 log10 TCID50/ml for vaccine with stabilizing medium WBM and an average loss
of 0.4 log10 TCID50/ml for vaccine with stabilizing medium PPNZ. The results favored the choice of
PPNZ Stabilizers on viability of 75/1 PPR virus during freeze-drying process and preservation at -20◦C
and -70◦C. Vaccine with stabilizing medium WBM and PPNZ stored at 4◦C for 12 months maintained
sufficient viable PPR virus to protect goat. At room temperature of 25C, the two types of vaccine
sufficient viable virus up to 9 months and loss their titers by 19th day of storage at 37◦C. Higher mean
neutralizing PPR antibody titers induced by vaccine with stabilizing medium PPNZ than that produced
by vaccine with stabilizing medium WBM in goats. In conclusion PPNZ is very effective in the
stabilization of PPRV and it is superior to WBM in inducing protective immune response after
vaccination of goats with no significant difference between them

Keywords

Benha Veterinary Medical Journal
Volume 29, Issue 1
September 2015
Pages 183-188

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