Evaluation of inactivated Rift valley fever virus vaccine adjuvanted with montanide oil ISA 201 VG

Document Type : Original Article

Authors

1 Department of Virology, Faculty of Veterinary Medicine, Benha University

2 Department of RVf, veterinary serum and vaccine research institute, Abbassia, Cairo

Abstract

Inactivated tissue culture adapted Rift Valley Fever (RVF) Virus vaccines were prepared using
Montanide oil ISA 201 VG and aluminum hydroxide gel as adjuvants. The prepared vaccines were
sterile and safe inducing no systemic or local clinical signs in sheep. Comparative evaluation of
experimentally prepared vaccines in sheep after a single dose indicated that the oily prepared vaccines
stimulated the humeral immune response as estimated by SNT and ELISA compared with aluminum
hydroxide gel vaccine. Protective serum antibody titers against in Montanide oil ISA 201 VG
adjuvenated RVF vaccine appeared in protective level at 1st week post vaccination and aluminum
hydroxide gel adjuvenated RVF vaccine at 2nd week. This protective titer persisted till 12th month in
the new oily vaccine and till the 6th month for Aluminum hydroxide gel vaccine. Both of SNT and
ELISA indicated that among the used adjuvant Montanide oil ISA 201 VG was preferable

Keywords

Benha Veterinary Medical Journal
Volume 29, Issue 1
September 2015
Pages 220-223

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