Comparative evaluation of different inactivated Rift valley fever virus vaccine adjuvanted with montanide oil ISA 61 VG, montanide oil ISA 201 VG, and aluminum hydroxide gel

Document Type : Original Article

Authors

1 Department of Virology, Faculty of Veterinary Medicine, Benha University

2 Department of RVf, veterinary serum and vaccine research institute, Abbassia, Cairo

Abstract

Inactivated tissue culture adapted Rift valley fever (RVF) virus vaccines were prepared using Montanide
oil ISA 61 VG, Montanide oil ISA 201 VG and aluminum hydroxide gel as adjuvants. The prepared
vaccines were sterile and safe inducing no systemic or local clinical signs in sheep. Comparative
evaluation of experimentally prepared vaccines in sheep after a single dose indicated that the oily
prepared vaccines stimulated the humeral immune response as estimated by SNT and ELISA compared
with aluminum hydroxide gel vaccine. Protective serum antibody titers against in Montanide oil ISA
201 VG adjuvenated RVF vaccine appeared in protective level at 1st week post vaccination but it
appeared as protective in Montanide oil ISA 61 VG adjuvenated RVF vaccine at 2nd week and in
aluminum hydroxide gel adjuvenated RVF vaccine at 2nd week. These protective titers persisted till
12th month in the new two oily vaccines and till the 6th month for Aluminum hydroxide gel vaccine.
Both of SNT and ELISA indicated that among the used adjuvants Montanide oil ISA 61 VG and
Montanide oil ISA 201 VG was preferable.

Keywords

Benha Veterinary Medical Journal
Volume 29, Issue 1
September 2015
Pages 224-228

Files

Share

How to cite

Statistics