Study on Inactivated Equine herpesvirus-1 Vaccine Adjuvanted with Montanide Pet Gel A

The study reported here is a part of a long-term research effort designed in our lab to select the best
adjuvant, which can be used with Equine herpesvirus-1 (EHV-1) inactivated vaccine to induce good
immune response in horses without any undesirable post vaccinal reaction. This study was carried out
on Montanide pet gel A, which is an innovative adjuvant developed for companion animals. To evaluate
the safety and potency of EHV-1 inactivated vaccine adjuvanted with Montanide pet gel A, It was
inoculated into Guinea pigs as a preliminary evaluation, given mean complement fixing antibody titers
of 67 and 176 at 21and 35 days post inoculation (dpi) respectively, and mean ELISA antibody titers of
680 and 1322.5 were recorded at 21and 35 dpi respectively, There was no any undesirable post vaccinal
reaction in horses inoculated with the prepared vaccine. The immune response of the inoculated horses
was monitored up to eight months post inoculation (mpi), Found that the peak of complement fixing and
ELISA antibody mean titer was 213.3 and 1335 at 2.5 and 3 mpi respectively. ELISA antibodies
persisted up to 6 mp. In conclusion, the prepared EHV-1 inactivated vaccine adjuvanted with montanide
pet gel A is highly safe and potent for Horses.