A total of 100 random samples of vacuum packaged meat products represented by sausage ̦ luncheon ̦ frunkfurter and salami (25 of each) were collected from different supermarkets in Cairo, Giza and Kalyobia governments. The prevalence of total anaerobes was 84%with account ranged between<10and 5.38 with a mean 3.58±0.36 for sausage, 56% with account ranged between<10and 3.92 with a mean value 1.76±0.33 for salami total anaerobes in and 60% with account ranged between <10 and 5.73 with a mean 2.55±0.44for luncheon. The incidence of Clostridia perfringens isolated from the examined samples of vacuum packaged salami, sausage, luncheon and frunkfurter was 44%, 80%, 32% and 0% respectively. The enterotoxins of Clostridia perfringens isolated from the examined meat products by traditional methods were type A and type C. Agarose gel electrophoresis of multiplex PCR of specific primers for characterization of enterotoxins (A, C & D) of C. perfringens indicated that sausage had enterotoxin type A and enterotoxin type C, luncheon had enterotoxin type A, enterotoxin type C and enterotoxin D, however salami had enterotoxin type A and type D
Ibrahim, H., Amin, R., El-Shater, M., & Mohammed, M. (2015). Detection of C. perfringens toxins in vacuum packaged meat products by using polymerase chain reaction. Benha Veterinary Medical Journal, 28(2), 67-73. doi: 10.21608/bvmj.2015.31868
MLA
Hemmat M. Ibrahim; Reham A. Amin; Mohammed A. El-Shater; Marwa M. Mohammed. "Detection of C. perfringens toxins in vacuum packaged meat products by using polymerase chain reaction". Benha Veterinary Medical Journal, 28, 2, 2015, 67-73. doi: 10.21608/bvmj.2015.31868
HARVARD
Ibrahim, H., Amin, R., El-Shater, M., Mohammed, M. (2015). 'Detection of C. perfringens toxins in vacuum packaged meat products by using polymerase chain reaction', Benha Veterinary Medical Journal, 28(2), pp. 67-73. doi: 10.21608/bvmj.2015.31868
VANCOUVER
Ibrahim, H., Amin, R., El-Shater, M., Mohammed, M. Detection of C. perfringens toxins in vacuum packaged meat products by using polymerase chain reaction. Benha Veterinary Medical Journal, 2015; 28(2): 67-73. doi: 10.21608/bvmj.2015.31868