Synergistic effect between some antimicrobial agents and rosemary (rosmarinus officinalis) toward staphylococcus aureus – in-vitro

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Dep., Faculty of Vet. Med., Benha Uni.

2 Animal Health Research Institute Dokki, Giza

3 Veterinarian

Abstract

The present study was carried out to evaluate the antimicrobial activity of ethanol plant extract of
rosemary (Rosmarinus officinalis) and / with five antimicrobial agents of different mechanisms
(oxytetracycline Hcl, amoxicillin, cefquinome, sulphaquinoxaline and danofloxacin) against field strain
of S. aureus. By using agar well diffusion method, the mean zone of inhibition (mm) of ethanol extract
of Rosemary were 21.67±0.33, 20.33±0.33, 19.67±0.17, 18.83±0.17, 18.17±0.17, 16.33±0.33 and
11.5±0.29 mm at different concentrations. While the mean zone of inhibition (mm) of different
antimicrobial agents at different concentrations for amoxacillin was 24±0.29, 20.67±0.33, 17.67±0.33
and 14.17±0.17mm and for cefquinome was 20.83±0.17, 18±0.29 and 13.5±0.29 mm. no inhibition
zones were detected with danofloxacin, sulphaquinoxalline and oxytetracycline Hcl. By macrodilution
method, the MIC of rosemary was 0.048mg/ml and the MICs for the aforementioned antimicrobial
agents were 0.015, 0.25, 1.0, 8.0 and 16 μg/ml respectively. The synergistic effects were recorded by
FIC index between 0.006 and 0.00038 mg/ml of rosemary with 0.125 and 4.0 μg/ml of cefquinome and
sulphaquinoxalline respectively.

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