A total of 150 random samples were collected from different supermarkets and retailers of different sanitation levels at Gharbeia governorate, Egypt as follows 70 samples of minced meat, 40 samples of sausage and 40 samples of beef burger. The aforementioned samples were subjected to bacteriological and serological applications to assess the prevalence of S. Enteritidis. The obtained results revealed that the incidence of S. Enteritidis in the examined samples of minced meat, sausage and beef burger were 1/70 (1.4%), 1/40(2.5%) and 0/40(0%) respectively. The isolates were submitted for serological analysis and revealed that Salmonella Enteritidis O 1,9,12 ad monophasic H:g, m. The antibiogram sensitivity test was applied upon the two isolates of S. Enteritidis and revealed that they are sensitive to chloramphinicol, amoxicillin, levofloxacin, ciprofloxacin, enrofloxacin and gentamycin but they were resistant to oxytetracycline. The virulence genes of S. Enteritidis isolates were determined by using multiple PCR technique for the two serologically detected Salmonella Enteritidis by using the following genes inv A, sef A, sop B and bcf C at 284bp, 310bp, 517bp, 467bp, respectively.
Abd El-Tawab, A., El-Hofy, F., Alekhnawy, K., & Sharaf, D. (2015). Detection of salmonella enteritidis in some meat products by using PCR. Benha Veterinary Medical Journal, 28(2), 202-207. doi: 10.21608/bvmj.2015.32503
MLA
Ashraf A. Abd El-Tawab; Fatma I. El-Hofy; Khalid I. Alekhnawy; Doaa M. Sharaf. "Detection of salmonella enteritidis in some meat products by using PCR". Benha Veterinary Medical Journal, 28, 2, 2015, 202-207. doi: 10.21608/bvmj.2015.32503
HARVARD
Abd El-Tawab, A., El-Hofy, F., Alekhnawy, K., Sharaf, D. (2015). 'Detection of salmonella enteritidis in some meat products by using PCR', Benha Veterinary Medical Journal, 28(2), pp. 202-207. doi: 10.21608/bvmj.2015.32503
VANCOUVER
Abd El-Tawab, A., El-Hofy, F., Alekhnawy, K., Sharaf, D. Detection of salmonella enteritidis in some meat products by using PCR. Benha Veterinary Medical Journal, 2015; 28(2): 202-207. doi: 10.21608/bvmj.2015.32503