Differentiation between streptococcus agalactia, streptococcus dysagalactia and staphylococcus aureus isolated from milk of mastitic cows by polymerase chain reaction

Document Type : Original Article

Authors

1 Animal Health Research Institute، Shibin El Koom branch, Department of Bacteriology

2 Animal Health Research Institute، Shibin El Koom branch, Department of Food Hygiene

Abstract

This study was conducted for bacteriological examination of 130 milk samples collected from clinically
mastitic cows in different areas at Menoufyia governorate . The results showed that streptococcus
agalactia ،streptococcus dysagalactia and staphylococcus aureus were isolated with percentage (11.5,
2.3% an 10%) respectively. Polymerase Chain Reaction (PCR) using specific primer were used as an
accurate and cost- effective molecular diagnostic technique of identifying mastitis pathogens

Keywords