Molecular characterization of IBD virus isolated from Giza governorate, Egypt, 2014

G.F., El-Bagoury; A. S., El-Habbaa; El-Sayed, Nada A. F.; A., Abu-Zied A.

doi:10.21608/bvmj.2015.32750

Molecular characterization of IBD virus isolated from Giza governorate, Egypt, 2014

Document Type : Original Article

Authors

¹ Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt

² Veterinary Serum and Vaccine Research Institute, Abassia, Cairo

10.21608/bvmj.2015.32750

Abstract

The emergence of new variant strains of the infectious bursal disease virus (IBDV) has been the cause of
serious economic losses in chicks. A field isolate of IBDV was recovered from suspected broiler chicks in
different localities of Giza governorate in 2014. The virus was isolated on specific pathogen free -
embryonated chicken eggs (SPF-ECE), detected by AGPT and confirmed by RT-PCR searching for VP2
coding region of the serotype I IBDV. A total number of 26 out of 42 tested samples (61.9%) were positive
for virus isolation, AGPT and RT-PCR. Sequence analysis of the PCR product and genomic characterization
of the variable region of VP2 of the field isolate revealed amino acid substitutions in the hydrophilic region
A in the loops containing domains important for antigenic variation, binding of neutralizing antibodies and
the residues responsible for the viral virulence and cellular tropism. Studying homology and phylogeny of
the isolated virus with the other reference classical, very virulent, variant and vaccinal strains of IBDV
revealed that IBDV Giza 2014 was in a separate branch and it was clustered more close to the Egyptian very
virulent IBDV, Giza 2000 and Giza 2008 and the European and German IBDV but it was clustered at a far
distance from Hel 2008 IM, Hel 2008, Bursine Plus and other strains. It was concluded that genomic
characterization of a variant IBDV which denote the continuous evolution and mutation of IBDV in Egypt
which may affect the virus antigenicity and virulence

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