Ameliorative Effect of Novel Nanocompound against Oxidative Stress- Induced Brain Toxicity in Mice

Document Type : Original Article


1 Biochemistry and Molecular Biology Department, Faculty of Veterinary Medicine, Benha University, Benha, Al Qalyubiyah, Egypt

2 Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, P.O. Box 13759, Egypt. Molecular Oncology Department - Cancer Institute (WIA), 38 Sardar Patel Road

3 Biochemistry and Molecular Biology Department, Faculty of Veterinary Medicine, Benha University


This study investigates the antioxidant properties of pH-responsive chitosan-based poly (acrylamide/acrylic acid) hesperidin (Cs/P(AAc/AAm)/HSP) Nanogel in AlCl3+NaF-intoxicated mice brain tissues as a pre-treatment and post-treatment agent. Fifty adult male Albino mice were used in our study, and were divided equally into 5 groups of (Ⅰ) normal control, (Ⅱ) Cs/P(AAc/AAm)/HSP, (Ⅲ) AlCl3+NaF, (Ⅳ) pre-treatment (prophylaxis), (Ⅴ) post-treatment (therapeutic). We used the colorimetric technique to detect the concentrations of MDA and GSH as well as the activity of SOD and GPx. AlCl3+NaF induced severe neurotoxicity and oxidative stress in mice brain tissue, manifested by the marked changes in the studied parameters. Either pre- or post-Cs/P(AAc/AAm)/HSP oral administration ameliorated these changes, as demonstrated by the significant reduction in MDA concentration, and increased GSH levels. Additionally, Cs/P(AAc/AAm)/HSP restored the activity of SOD and GPx enzymes, indicating mitigation of the induced oxidative stress. Our findings from this study clearly showed the antioxidant potential of Cs/P(AAc/AAm)/HSP either as a prophylactic or therapeutic agent against AlCl3+NaF-induced brain damage.


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