Preparation and evaluation of a novel combined inactivated vaccine against Pasteurellosis and E. coli infection in Sheep

Document Type : Original Article


1 Aerobic Bacteria Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt

2 Central laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), 131, Cairo, Egypt


Pneumonic Pasteurellosis is a common disease in sheep that affect the sheep industry. Also, E. coli, which affects young lambs and adult sheep, causing morbidity and mortality, threatens the sheep's wealth. Therefore, an inactivated adjuvanted combined vaccine against pasteurellosis and E. coli was prepared to counteract their catastrophic effects.
Twenty healthy sheep aged 6 weeks were divided into four groups. The first was immunized with the P. multocida (A, D, B6) and M. hemolytica (A, P. trehalosi type T) vaccine. The second was immunized with the E. coli (K99) vaccine. The third was immunized with a combined P. multocida, M. hemolytica, and E. coli vaccine. The fourth was kept as a control group. The sheep were injected subcutaneously with two doses (2 weeks apart) of the vaccines (1 ml/dose). The vaccination was at 6 weeks of age. The highest antibody titers after boostering for groups vaccinated with P. multocida and M. hemolytica vaccines were as follows: the 1st group and the 3rd group for type A, B6, and P. trehalosi at the 4th month, but P. multocida type D at the 5th month and M. hemolytica type A at the 3rd month. The 2nd group and the 3rd group had the highest antibody titers in the 3rd month. Statistically, there were no significant differences between the results of the combined or single vaccines. Briefly, the combined inactivated vaccine against pasteurellosis and E. coli has good effects, with priority given to decreasing the stress on the livestock and the workers.


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