Molecular characterization of lumpy skin disease virus in specific Egyptian governorates using ORF 103 sequencing during early 2023

Document Type : Original Article

Authors

1 1Virology Department, Faculty of Veterinary Medicine, Benha University, Egypt. 2Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza, Egypt.

2 Virology Department, Faculty of Veterinary Medicine, Benha University, Egypt.

3 Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza, Egypt.

Abstract

In April and May 2023, five skin nodule samples were collected from cattle suspected to have lumpy skin disease (LSD) in three governorates in Egypt: Dakahlia, El-Menia, and El-Fayoum. Lumpy skin disease virus (LSDV) was detected quickly and specifically using Taq-Man real-time PCR. All samples proving positive for the virus. Following that, one positive sample from El-Fayoum governorate was isolated on Madin-Darby bovine kidney (MDBK) tissue culture for three passages, and characteristic LSDV cytopathic effects (CPE) were observed. To molecularly characterize and differentiate the strain, the isolated sample was subjected to sequencing and phylogenetic analysis, with a focus on the partial sequence of ORF 103. The results showed that the isolated virus belonged to the field strain group of LSDV, which was separate from vaccinal strains, demonstrating that ORF 103 sequencing data can be used to differentiate between field wild strains and vaccinal strains, as well as other Capripoxviruses (CaPVs).

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Benha Veterinary Medical Journal
Volume 45, Issue 2
December 2023
Pages 163-167

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