Evaluation of different adjuvants to the inactivated PPR vaccine

Hussein, Manal Abdelaziz; Elbagory, Gabr Fikry; Khodier, Mohammad Hassan

doi:10.21608/bvmj.2023.247497.1747

Evaluation of different adjuvants to the inactivated PPR vaccine

Document Type : Original Article

Authors

¹ 1 Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt 2 Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt

² Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt

³ Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt

10.21608/bvmj.2023.247497.1747

Abstract

The Peste des petits ruminant (PPR) is a viral devastating illness of sheep and goats resulting in huge losses to the economy and its control mainly depends on successful vaccination of susceptible hosts. The use of inactivated vaccine is preferable in non-endemic regions. Determination of the best adjuvant to the inactivated PPR vaccine induces the highest vaccine potency. Preparation of inactivated PPR virus infected fluid obtained from infected Vero cell by binary ethylene amine and divided it into three portions adjuvant by Montanide -01 gel; Montanide oil ISA 206 and Carbomer then subjected to quality control testing. All of the three prepared inactivated PPR vaccine formulae were found to be free from foreign contaminants; safe and highly immunogenic inducing nearly similar antibody titers in vaccinated sheep remaining up to 28 weeks post vaccination and suggested to be of longer duration. Each of Mantonide-01 gel; Montanide oil ISA 206 and Carbomer could be recommended to use as an adjuvant to the inactivated PPR vaccine with regard to the cost of vaccine production.

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