Potency of oil adjuvant inactivated Peste des Petits Ruminants (PPR) vaccine

Hussein, Manal Abdelaziz; Elbagory, Gabr Fikry; Khodier, Mohammad Hassan

doi:10.21608/bvmj.2023.247445.1746

Potency of oil adjuvant inactivated Peste des Petits Ruminants (PPR) vaccine

Document Type : Original Article

Authors

¹ 1 Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt 2 Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt

² Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt

³ Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt

10.21608/bvmj.2023.247445.1746

Abstract

The Peste des petits ruminant (PPR) is a viral disease caused by a morbilli virus resulted in huge economic losses among sheep and goats. Vaccination is the essential corner stone to control it. Preparation of a safe potent inactivated vaccine could be used during outbreaks or in enharbouring countries free from the disease. Preparation of PPR virus infected fluid by virus inoculation on Vero cell line followed by virus titration and inactivation by binary ethylene amine and adjuvant by Montanide oil ISA 206 followed by application of quality control testing on the final preparation (freedom from foreign contaminants; safety and potency in susceptible sheep). The prepared inactivated PPR vaccine adjuvanted with Montanide 206 oil is safe and potent able to provide vaccinated sheep with high protective levels of specific immunity. The inactivated PPR vaccine can be used for immunization of sheep protecting them against the virus infection especially those received 2 doses on 2 weeks interval. It is suggested that the obtained immunity may be of long duration remain up to 12 months post vaccination.

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