Use of Carbopol as an adjuvant in preparation of inactivated rabbit pasteurellosis vaccine

Document Type : Original Article

Authors

1 Aerobic Bacterial vaccines Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt

2 Department of pet animal vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt

Abstract

The current study was designed to investigate the immunological response of rabbits vaccinated with the rabbit pasteurellosis vaccine adjuvanted with Carbopol®. One hundred and twenty, 2 weeks old rabbits were divided into four groups, 30 for each. The first group was received the formalized P. multocida vaccine. The second group was received the Montanide ISA206 P. multocida vaccine. The third group was received the Carbopol P. multocida vaccine. The fourth group was left unvaccinated as a control group. The prepared vaccines were evaluated by the measurement of the antibody response by the indirect haemagglutination (IHA) and ELISA techniques. The vaccines' potency was assessed using the challenge test against the virulent strains of P. multocida serotypes A and D. The findings revealed that the Carbopol® rabbit pasteurellosis vaccination elicited a strong and long-lasting antibody response, as well as a considerable protection against the virulent strains of P. multocida serotypes A and D.

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