Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.

Document Type : Original Article

Authors

1 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor

2 Department of Food Hygiene, Animal Health Research Institute, Tanta Branch, Agricultural research center (ARC)

3 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

Abstract

Non-typhoidal Salmonellosis is a major foodborne infection. The objectives of our study were to investigate the prevalence rates of multiple drug resistant (MDR ) Salmonella spp in various food products, A total number of 100 food samples including 20 samples for each of kariesh cheese ,raw milk , chicken fillet and minced meat and 10 samples for each of chicken liver and raw meat were collected from February to July of 2022 within different markets in El-Gharbia,Egypt. All samples were subjected for isolation and identification of Multiple drug resistant Salmonella by cultivation on xylose lysine deoxycholate (XLD) agar , biochemical test ,serological test , antibiograms and the Polymerase Chain Reaction (PCR) screen of virulence gene (invA ) ,class 1 integron and resistant genes (blaTEM ,qnrS and aadA1). The incidence of Salmonella was 2(2%) and based on molecular serotyping were identified as S. Kentucky and S.Typhimurium . All Salmonella isolates (100%) showed MDR . Variable antibiotic resistance patterns were detected from 100% for Ampicillin ,norfloxacin and gentamicin to 50% for Doxycycline and ciprofloxacin. Based on PCR analysis, invA, class (1) integron ,blaTEM, qnrs and aadA1 were detected in (100% ) of all identified Salmonella isolates .

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