Phenotypic and Genotypic Characters onAeromonas Species Causing Mortality in Fishes

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Dept., Fac.Vet. Med., BenhaUniv.

2 El-Abbassa central laboratory of Aquaculture Research, Sharkia Governorate, Egypt.

3 Vet. Researcher

Abstract

This study was carried out to identify aeromonas species causing mortalities in some fishes. Atotal number of 102 Nile tilapia fish( Oreochromisniloticus) and 45 common carp (Cyprinuscarpio) all had clinical signs and collected from El-Abbassa fish farms and subjected to full clinical, post mortem and bacteriological examinations.The present study revealed,The most prevailing bacterial diseases affecting Nile tilapia and Common carp were motile Aeromonas(A.sobria, A.veronei, A.jandae, A.hydrophilaandA.cavae) The pathogenicity test revealed that mortality rate was 20 – 100 % in Nile tilapia fish due to A.sobria experimental infection. Some isolates of A.veronei and A.hydrophila were pathogenic to Nile tilapia.Aerolysin and Ahcytoen genes were detected in all isolates of A.sobria subjected to PCR.Resistance genes (qnrs and blaTEM) were also detected in all isolates of A.sobria subjected to PCR. While ermBwas negative for all isolates. A.sobria was sensitive to ciprofloxacin, norfloxacin and trimethoprim and resistant to amoxicillin, erythromycin, gentamycin, rifamycin, bacitracin and cephradine

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