Efficacy of an inactivated bivalent Rabbit Hemorrhagic Disease Virus 2 – Pasteurellosis vaccine in Rabbits by using a new Adjuvant.

Document Type : Original Article

Authors

1 Poultry viral disease, Veterinary Serum and Vaccine Research Institute, Agriculture research center.

2 Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Cairo, Egypt

3 Veterinary serum and vaccine research institute, Agricultural Research Center (ARC),Cairo, Egypt

Abstract

Rabbit production is an important investment in the animal sector.The purpose of this study is to prepare an efficient vaccine combating Rabbit Hemorrhagic Disease Virus (2) (RHDV2) and Pasteurellosis (serotypes A:1, A:3, A:12, D:2) using Coral vac RZ 528 adjuvant. Four groups of rabbits were used to evaluate the efficiency of this produced vaccine. Groups were injected as follow: 1 with RHDV2 vaccine, 2 with P. multocida vaccine, 3 with the adjuvanted combined vaccine and 4 was kept as a control group . The efficiency of the prepared vaccines was assessed through Hemagglutination Inhibition (HI) and indirect Hemagglutination Inhibition (IHA) , as well as challenge tests for RHDV2 &P. multocida. The HI test results explored that, 12th week after vaccination was the peak in the antibodies titers (11.5 for RHDV vaccine and 11.8 for the combined one). The challenge test showed that both the vaccines were 100% protective. For P. multocida and the combined vaccines, the evaluation were done by IHA test and challenge test. By IHA test, the 6th week was the highest in the antibodies titers (for P. multocida vaccine 1638.4 for A, 1433.6 for D serotypes and for the combined one 2048 for A, 1843.2 for D). The challenge test result was 90% protection rate for both vaccines. Briefly, the combined prepared vaccine is efficient and protective against both diseases with the advantages of decreasing the stressors on the animals and the workers and lowering the cost.

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