Effects of pomegranate peel extract-coated zinc nanoparticles on antioxidant status, biochemical parameters, and pathological findings in broiler chickens infected by avian pathogenic E. coli

Hegazy, Yomna Abdel Rahman; Elsenosi, Yakout; Aziza, Samy; Abouzaid, Omayma Ahmed; Fotouh, Ahmed

doi:10.21608/bvmj.2025.404025.1969

Effects of pomegranate peel extract-coated zinc nanoparticles on antioxidant status, biochemical parameters, and pathological findings in broiler chickens infected by avian pathogenic E. coli

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.

² 2Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, New Valley University, Egypt. 3Department of Business Administration, Marywood University, Scranton, PA

10.21608/bvmj.2025.404025.1969

Abstract

E. coli infection (colibacillosis) is among the most prevalent and significant poultry diseases due to its role in causing decreased weight gain, mortality, and decreased flock uniformity, though E. E. coli as a normal flora can be present in birds' intestinal tracts; some strains may cause respiratory and systemic diseases, referred to as avian pathogenic Escherichia coli (APEC). The objectives of the study were to evaluate the growth performance and physiological parameters of E. coli-infected chickens treated with pomegranate extract-loaded zinc nanoparticles (PPE-ZnO-NPs). One hundred and fifty broiler chickens were allotted into five groups, consisting of 30 birds per group. In group G1, the control birds were negative; in group G2, the control-positive infected birds were positive for E. coli O87; G3, the infected birds were supplied with drinking water containing 40 mg/L of PPE-ZnO-NPs; G4, the infected-treated with 80 mg/L of PPE-ZnO-NPs given by drinking water; G5, the infected-treated with 120 mg/L of PPE-ZnO-NPs given for 35 days. The bird performance and mortalities were monitored, and blood and tissue samples were collected for biochemical, antioxidant, and pathological analysis. The mean of serum total cholesterol, triglycerides, creatinine, and urea was significantly reduced (P<0.05) by giving PPE-ZnO-NPs. The activity of malondialdehyde (MDA) was significantly decreased (P<0.05), while superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were significantly increased (P<0.05) by the addition of PPE ZnO-NPs compared to the positive control. It could be concluded that supplementation of PPE-ZnO-NPs to broiler improved birds' physiological and antioxidant status, which were infected with E.coli.

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