Numerous of Cases of skin nodules eruptions tied to lumpy skin disease virus (LSDV) are becoming more frequent in Qailubia Province, Egypt amongst cattle populations in 2014. In this study, a strain of LSDV (LSDV-Elkady-2014) associated with that outbreak was isolated from scabs of clinically infected Frisian cattle on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken eggs (SPF-ECEs). The isolate induced hemorrhage, enlarged liver, slight heart hypertrophy of chick embryos, with a pock lesion on CAM. Identification of LSDV-Elkady-2014 isolate was confirmed by histopathology, electron microscopy (EM), immunoflourescence (IF), and polymerase chain reaction (PCR) targeting LSDV envelope protein-like gene. Based on the partial sequence analysis of LSDV envelope protein-like gene. LSDV strain was phylogenetically related to LSDV- NI-2490, LSDV/ NW/LW, and LSDV/2/slemani/Kurdistan/ 2014. In conclusion, LSDV is still circulated in Egypt, and the isolated strain induced characteristic properties on chick embryos that were not previously recorded by LSDV.
El-kady., G., El-Bagoury, G., El-Nahas, E., & El-Habbaa, A. (2018). A Phylogeny of Envelope protein like gene of Lumpy skin disease virus, Egypt, 2014. Benha Veterinary Medical Journal, 34(3), 338-354. doi: 10.21608/bvmj.2018.47868
MLA
G.HE.M El-kady.; G.F. El-Bagoury; E.M. El-Nahas; A.S. El-Habbaa. "A Phylogeny of Envelope protein like gene of Lumpy skin disease virus, Egypt, 2014", Benha Veterinary Medical Journal, 34, 3, 2018, 338-354. doi: 10.21608/bvmj.2018.47868
HARVARD
El-kady., G., El-Bagoury, G., El-Nahas, E., El-Habbaa, A. (2018). 'A Phylogeny of Envelope protein like gene of Lumpy skin disease virus, Egypt, 2014', Benha Veterinary Medical Journal, 34(3), pp. 338-354. doi: 10.21608/bvmj.2018.47868
VANCOUVER
El-kady., G., El-Bagoury, G., El-Nahas, E., El-Habbaa, A. A Phylogeny of Envelope protein like gene of Lumpy skin disease virus, Egypt, 2014. Benha Veterinary Medical Journal, 2018; 34(3): 338-354. doi: 10.21608/bvmj.2018.47868
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