Comparative immune evaluation of inactivated Derzsy virus disease vaccine in SPF chicken and Muscovy duck

Document Type : Original Article

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Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Abbasia, Cairo Egypt.

Abstract

 Objective: Assessment the efficacy of commercial inactivated duck parvovirus vaccine in conventional ducklings by monitoring the antibody response of domestic Muscovy ducks after subcutaneous immunization and comparing the humoral immune response between vaccinated Muscovy ducklings and vaccinated SPF chicks using ELISA. Two separate vaccination experiments, here after referred to as experiments A and B, with an identical experimental design were performed to study the efficacy of different three batches of commercial inactivated Parvovirus vaccine against Derzsy’s disease.
Methods: In the first experiment (A): One hundred and five susceptible Muscovy ducks one week old  were vaccinated  with the recommended dose (0.2 ml/ bird) via subcutaneous route (S/C) (twenty ducks for each batch) and fifteen ducks were kept without vaccination as a non- vaccinated control. Another group of ducks (ten ducks for each batch) were vaccinated with double the recommended vaccination dose via (s/c) and served as safety group. 
In the second experiment (B): One hundred and five specific pathogen free chicks of three weeks old were vaccinated following the same procedures the vaccine was injected with 0.2 ml/bird subcutaneously in the dorsal region of the neck (twenty chicks for each batch). Other fifteen chicks were kept in separate isolator as non-vaccinated negative control. Another group of chicks (ten SPF chicks for each batch) were vaccinated with double the recommended vaccination dose via S/C and served as safety group. 
Results:  The monitoring of the humeral immune response of both vaccinated waterfowl and SPF chicks were estimated by enzyme-linked ImmunoSorbant assay (ELISA). Comparing the humeral immune response of both vaccinated waterfowl and SPF chicks by ELISA showed that protective serum samples for GPV/MDPV. Results of serum samples of four weeks post vaccinated Muscovy ducks and SPF chicks were positive for different three vaccine batches GPV/MDPV antibodies of four weeks PV.
Conclusion: The present study findings underlines that Muscovy ducks are the susceptible host for parvovirus up to approximately six weeks of  age while SPF chicks is non susceptible host for parvovirus and we used it as a model for production of humeral antibodies.
 

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