Bacteriological and molecular studies on Salmonella species isolated from poultry farms

Abd El-Tawab, Ashraf; Abdelbaset, Enas; Hegazy, Abd Elhalim; Abd-Elmonem, Rania

doi:10.21608/bvmj.2019.114673

Bacteriological and molecular studies on Salmonella species isolated from poultry farms

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Veterinary Medicine, Benha University, Egypt

² Department of Poultry Disease, Animal Health Research Institute, Agriculture Research Center Giza, Egypt.

10.21608/bvmj.2019.114673

Abstract

This study comprise the examination of 100 samples of feed( 40), water (36) and litter (24) collected randomly from different chicken shops, houses and farms in Kafr El-Sheikh Governorate. Results of this survey revealed isolation of 14/100(14%) Salmonella species (3)(12.5%) isolates from litter, (5)(13.8%) isolates from water and (6)(25%) isolates from farms . The prevalence rate to Salmonella was (14%) Sensitivity test was made and isolates exhibit resistance against ciprofloxacin and cefotaxime with (89%) and highly sensitivity to amikacin with (100%) so amikacin is the first drug choice for treatment of Salmonella infection. A PCR based assay was developed to developed to detect the prevalence of Salmonella in samples and to evaluate plasmid and chromosome-borne virulence genes (stn-invA) which considered as a target genes for the detection of Salmonella The results of detection of resistance genes (qnr S-blaTEM) as a resistance genes in all isolates of Salmonella.

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