DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes

Ramadan, Sherif; Saker, Abdel-Aziz; Shafik, Basant

doi:10.21608/bvmj.2020.34139.1221

DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes

Document Type : Original Article

Authors

¹ Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Egypt

² Department of biotechnology, Animal production Research institute, Agriculture Research Center, Cairo, Egypt

10.21608/bvmj.2020.34139.1221

Abstract

The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle Stimulating Hormone Receptor gene (FSHR) by using RFLP and direct sequence methods, also to investigate their possible association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were investigated. Animals were divided into three groups: normal fertile (20 animals), infertile due to anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic DNA was extracted from blood samples, then PCR was performed with annealing temperatures of 57ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each buffaloes’ group. It seems that FSHR gene are highly conserved in the investigated buffalo’s population.

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