Effect of chitosan and calcium phosphate nanoparticles on PPRV vaccine immunogenicity and keeping quality

Document Type : Original Article


1 Department of virology, Faculty of Veterinary Medicine, Benha University

2 Veterinary serum and vaccine Research institute, Abbasia, cairo.


Nanotechnology that created nanoparticles (NP) having superior properties compared to the original ones and offers a new era for vaccine improvement. In this study, Chitosan nanoparticle (CSN) and Calcium Phosphate nanoparticle (CaPN) based-vaccines were prepared in different concentrations [1% (W/V) & 0.125% (W/V)] then, trials to discover the effects of these NP based-Pest des Petits Ruminants (PPR) vaccines on the immunogenicity and keeping quality compared than that offered by the conventional PPR vaccine. The results revealed from the serum neutralization test (SNT) that performed on sera collected from inoculated sheep with NP based vaccines and conventional one on a weekly base interval for four weeks and with the same subcutaneously inoculated dose of 103 TCID50 showed that all NP based vaccines was faster in yielding neutralizing antibody titer from week one while conventional vaccine was delayed to the second week, meanwhile CSN 0.125% based vaccine was more superior than other NP based vaccines that reached the peak titer [Geometric Mean Titer (GMT) of 128] by the fourth week while conventional vaccine results were only showed 53.3 as GMT. These results confirmed that CSN and CaPN based vaccines were generally more superior than conventional one and CSN 0.125% based vaccine were achieved the forefront results. On the other hand, keeping quality tests based upon PPR virus titration of all types of vaccines in this trial that were exposed to different temperature degrees (4°c,25°c& 37°c) were also studied. The obtained results emphasized that  CSN 0.125% based vaccine superiority that showed only 0.4 log 10TCID50/ml, as a titer loss after lyophilization process, while the conventional vaccine lost 0.6 log 10TCID50/ml .On keeping all types of vaccines employed in this study at 4°c (for 12 weeks) , 25°c (for 12 weeks)  and 37°c (for 7 days) , it was found that CSN 0.125% based vaccine showed the lowest loss in titer among other NP based vaccines in our study, that was expressed by log 10TCID50/ml as 0.4,1.9 and 1.4 respectively. While loss of titer in case of conventional vaccine that kept at the same degrees of temperature was 0.5, 2.2 and 1.6 respectively. So, these results favored CSN 0.125% based vaccine that showed better results in immunogenicity and keeping quality rather than conventional PPR vaccine.


Main Subjects