Molecular studies on some factors of E. coli isolated from avian products and infants with diarrhea

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med., Benha Univ.

2 Animal Health Research, Tanta branch

Abstract

Extraintestinal pathogenic E. coli (ExPEC) are facultative pathogens that are part of the normal human intestinal flora which classified into several types, uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), sepsis-associated E. coli (SEPEC), and avian pathogenic E. coli (APEC) (Jolanta et al.,2019).
The present study was performed on a total number of 150 samples from which 100 samples were collected from poultry products (Gizzard, Liver, Banih, and Nuggets) also 50 samples from infants stool at Gharbia Governorate for isolation of E.coli and detection of some virulence genes.
The results revealed that 26 samples were positive for Escherichia coli, 11 from infants stool, 7 from poultry liver samples, 5 poultry gizzard , 2 Banih and 1 from Nuggets samples.
Amikacin 30 and ciprofluxacin 5 (oxoid) were the highest efficiency antimicrobial agents used in vitro.
Six E.coli isolates were serotyped and identified as O157, O128, O125 and O86a.
PCR results showed that TraT and pic pathotypic genes ( at 307 bp and 572 bp) were detected in all, but only O157 and O125 have stx2 gene ( at 779 bp).
All that indicate that E.coli strains isolated were extra-intestinal pathogenic strains.

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Benha Veterinary Medical Journal
Volume 39, Issue 2
December 2020
Pages 84-89

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