Beneficial effects of Omega-3 polyunsaturated fatty acids in treatment of non-alcoholic fatty liver disease in rats.

Hussein, Samy; Elsenosi, Yakout; Khalid, Mohamed; Fawzy Atta, Marwa Mahmoud

doi:10.21608/bvmj.2021.65003.1351

Beneficial effects of Omega-3 polyunsaturated fatty acids in treatment of non-alcoholic fatty liver disease in rats.

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.

² Department of Biochemistry , faculty of Veterinary medicine, Benha University,Egypt.

³ Department of Biochemistry, Faculty of Veterinary. Medicine., Benha University, Egypt.

⁴ Faculty of Vetrinary medicine ,Benha University

10.21608/bvmj.2021.65003.1351

Abstract

Nonalcoholic fatty liver disease (NAFLD) is sickness with different causes and it is the most hepatic illness. The objective of this work is to investigate the bimolecular changes in a model of high fat diet induced- NAFLD in rats. Also, the hypolipidemic, antioxidant and anti-inflammatory effects of omega-3 were clarify via evaluation of some biochemical and molecular parameters in the liver tissue of rats.Twenty one adult white albino rats were divided into three groups. Group I (Normal control): received no drugs and fed balanced diet, Group II (NAFLD- induced group): rats fed high fat diet for 8 weeks followed by normal basal ration for additional 8 weeks. Group III (NAFLD + omega-3): rats administrated omega-3 (300 mg/kg b.wt/day), orally for 8 weeks post-induction of NAFLD. The obtained results showed a significant increase in serum Total cholesterol and Triacylglycerols (TAG) concentrations, L-MDA and TAC in liver tissuewith significant up-regulation in CPT-1α, FAS, SREBP-1c, PPAR-α, Nrf2 and TAK1gene expression in NAFLD-induced rats. Treatment with Omega-3 to NAFLD induced rats potentially improved molecular hepatic cell function and oxidative alterations related to NAFLD near its normal ranges. Meanwhile, histo-pathological findings supported that Omeg-3 markedly attenuates harmful effects of NAFLD and protected liver cell. On conclusion, Omega-3 plays an important role as potent anti-oxidant, anti-obesity, anti-inflammatory via inhibition of hepatic steatosis and oxidative stress singling.

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