Spirulina platensis and Grape Seed Proanthocyanidin Extract ameliorates hepatic impairment in Carbimazole-induced hypothyroidism in male rats via caspase 8/Bcl-2 signaling pathway

Hussein, Samy Ali; Abdel Aal, Samir; Marzouk, Mohammed; Darweish, Marwa; Hussein, Asmaa Hamdi

doi:10.21608/bvmj.2022.149870.1556

Spirulina platensis and Grape Seed Proanthocyanidin Extract ameliorates hepatic impairment in Carbimazole-induced hypothyroidism in male rats via caspase 8/Bcl-2 signaling pathway

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.

² Department of Animal, poultry and environmental hygiene, Faculty of Veterinary Medicine, Benha University

³ Department of Pathology, Faculty of Veterinary Medicine, Benha University, Egypt

10.21608/bvmj.2022.149870.1556

Abstract

Spirulina and Grape Seed Proanthocyanin Extract (GSPE) were studied for their potential therapeutic and protective properties in hypothyroidism-induced rats. Six equal groups of forty-eight rats were formed. Group 1control Group 2 (hypothyroidism) received an oral dose of carbimazole (1.8 mg/kg b.wt.) every day.Rats in Group3 (GSPE Protected) got (150/kg b. wt) of GSPE per day.for the first 3 weeks, then continued administered with carbimazole for another 3 weeks. Group 4 (carbimazole+GSPEtreated): Rats were administered a daily dose of carbimazole (1.8mg/kg b.wt) for 3 weeks, followed by GPSE (150/kg b. wt/day) for another 3 weeks. Group 5 (Spirulina Protected);Rats received Spirulina (300 mg/kg body weight per day) for the first three weeks, then continued with carbimazole for another 3 weeks. Group 6 (Spirulina treated): Rats administered a daily dose of carbimazole for 3 weeks, followed by Spirulina (300/kg b. wt/day) for another 3 weeks. The results collected demonstrated a significant rise in liver Caspase-8 gene expression in the hypothyroidism group along with significant increases in blood TSH, total cholesterol, and triacylglycerols. However, serum T3, T4 levels drastically dropped along with Bcl2 expression in the liver tissue. Treatment and protection of spirulina and GPSE reduced TSH, total cholesterol, triacylglycerols and Caspase-8 with significant upregulation of Bcl-2 and marked decrease in total cholesterol and triacylglycerols concentrations.
In conclusion, grape seed extracts and Spirulina platensis had therapeutic potential in hypothyroidism, protect liver damage and mitigate Thyroid disruptors via anti-apoptotic activities.

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