Biochemical, histological and immune histochemical studies on the efficacy of garlic oil against thioacetamide induced hepatotoxicity in rats

Elsayed, Faten Ibrahim; Gad, Fatma Abdelmonem; Elkamar, Reda Ibrahim; Ahmed, Osama AbdelMohsen Salem

doi:10.21608/bvmj.2022.146236.1536

Biochemical, histological and immune histochemical studies on the efficacy of garlic oil against thioacetamide induced hepatotoxicity in rats

Document Type : Original Article

Authors

¹ Department of pharmacology.faculty of vet med.benha university

² Department of clinical pathology

³ Department of histology

⁴ department of Anatomy &amp; Embryology faculty of veterinary medicine , Benha university

10.21608/bvmj.2022.146236.1536

Abstract

Abstract
The main aim of this study is to verify the biochemical, histological and immunohistochemical efficacy of garlic oil against thioacetamide induced hepatotoxicity in rats. Thirty rats were used and divided into 5 groups I: The control group took 1 ml of isotonic saline orally once daily. 2: The garlic oil group is given orally 200mg/ kg body weight of garlic oil once daily. 3: The Thioacetamide (TAA) group was poisoned with TAA at a dose of 100 mg/kg body weight twice weekly. 4: The Garlic oil+ TAA group received garlic oil followed by an I/p injection TAA. 5: The Silymarin+TAA group took silymarin at a dose of 50 mg/kg body weight followed by TAA poisoning. All the treatment continued for 30 days. TAA-treated rats revealed dramatic increases in ALT, AST, ALP, TC and TG with a significant decline in TP, Alb, and HDL when compared to the control group. The TAA+garlic and TAA+silymarin groups, on the other hand, showed an improvement in the previous parameters. Oxidative/ antioxidant parameters demonstrated a significant reduction in the elevated level of MDA in garlic group than TAA-treated rats, Along with increased SOD, GSH and CAT activities were showed in TAA+garlic. Histopathological and immunohistochemical results showed TAA treated rats revealed evidence of obvious damage to the hepatic parenchyma with higher number of Bax immunopositive cells and down regulation of Bcl2 expression in cytoplasm of affected hepatocytes. But, garlic group reversed the toxic effect on liver. .

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