Document Type : Original Article
Authors
1
Animal and Poultry Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt
2
animal wealth development department, faculty of veterinary medicine, benha univeristy
3
Department of Animal Biotechnology, Animal production research institute, Agriculture research center, Egypt
4
Department of Buffalo Breeding, Animal production research institute, Agriculture research center, Egypt.
5
5Department of Theriogenology, Faculty of veterinary medicine Benha University, Egypt
6
6Animal hygiene and veterinary Management Department, Faculty of veterinary medicine Benha University,, Egypt
Abstract
This study's goal was to use the DNA sequence techniques to find new mutations in the follicle-stimulating hormone receptor exon 10 (FSHR) in Egyptian buffalo bulls with different sperm motility. A total of 50 Egyptian buffalo bulls aged 3–4 years old, at Kafr El-Sheikh Governorate (breeding station of Mahallet Mousa), Egypt were investigated. The bulls were divided into two groups according to spermatozoa motility: the first group of high sperm motility (> 60%, n = 32) and the second group of low sperm motility (< 60%, n = 18). Blood samples were collected to obtain genomic deoxyribonucleic acid, and to perform polymerase chain reaction with an annealing temperature of 57 °C to amplify 306 bp of the FSHR exon 10 gene. The results showed that all the investigated buffalo bulls were monomorphic and had the same genotype. We may conclude that there no different variants of FSHR gene associated with different sperm motility, therefore we need to study more genes in relation to sperm motility.
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