Determination of the optimal protective dose of inactivated Salmonella Typhimurium vaccine in pigeon

Document Type : Original Article

Authors

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt

Abstract

Salmonellosis is one of the major health problem affect pigeons and protection of pigeons against this disease is very
important, for this purpose the present work was planned to prepare an inactivated Montanide ISA- 206 oil adjuvant
vaccine from a locally pigeon isolate of Salmonella Typhimurium (S. Typhimurium) and determined of the optimal dose,
which protect pigeon against salmonellosis. Evaluation of such preparation following the quality control tests revealed
that it was free from any foreign contaminants, safe and immunogenic. Pigeons were divided into six groups, three of
them vaccinated subcutaneously with single dose (0.5ml) of different bacteria concentrations (108,109 and 1010 CFU/
dose) and the others three received 2 doses with 3 weeks intervals with same route and concentrations. The
vaccination/challenge assay with a virulent S. typhimurium organism using 5x107 CFU/dose revealed that protection rates
of vaccinated birds were 70% ,75% and 75% for the first three groups (Ia, IIa, IIIa) respectively, while the other three
groups gave 80%, 85% and 85% protection(Ib, IIb, IIIb) respectively.Control groups could not withstand the challenge
and protection rate were 15% and 20% respectively. The seroevaluation showed that the humoral immune response
developed against S. typhimurium in vaccinated pigeons was high. On conclusion the optimal dose of the prepared
vaccine should be 109 CFU/dose applied twice with three weeks interval, which can cover the needed requirements and
protect pigeon against salmonellosis

Keywords

Benha Veterinary Medical Journal
Volume 31, Issue 1
September 2016
Pages 73-77

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