Ameliorative effect of novel nanocomposite: basic curcumin nanoparticles modified with basic nano black seeds (nigella sativa) and calcium ascorbate on metabolic changes in experimentally induced tumor in female mice

Document Type : Original Article

Authors

Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt

Abstract

The ameliorative effect and antitumor activity of basic curcumin nanoparticles modified with basic nano
black seeds (nigella sativa) and calcium ascorbate were evaluated. eighty female mice were divided into
4 equal groups of 20 mice each. Group 1: non – tumor bearing mice (NTBM). Group 2: tumor bearing
mice(TBM). Group 3: NTBM + nanocomposite (4.750gm / kg. b.wt /day ) , orally for 6 weeks . Group
4: TBM + nanocomposite (4.750gm / kg. b.wt/day ), orally for 6 weeks . Blood samples for serum
separation and liver tissues specimens were collected from all animal groups three times after 2, 4 and
6 weeks from the onset of treatment. All serum was directly used for determination of ALT and AST
activities and urea and creatinine concentrations. Moreover, antioxidants enzymes (catalase, GPX,
SOD), GSH and MDA were detected in liver tissue. The obtained results showed significant decrease
in catalase, GPX and SOD activities and GSH concentration in liver tissue of tumor bearing mice.
Meanwhile, liver MDA, serum transferases(ALT and AST) activities, urea and creatinine concentrations
were significantly increased in tumor bearing mice . TBM treated with nanocomposite improve the
adverse biochemical changes induced by tumor. So, this nanocomposite has potential benefits in cancer
treatment

Keywords

Benha Veterinary Medical Journal
Volume 29, Issue 2
December 2015
Pages 235-244

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