Prevalence of E.Coli in broiler chickens in winter and summer seasons by application of PCR with its antibiogram pattern

Document Type : Original Article


1 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

2 Department of Animal, poultry and environmental hygiene, Faculty of Veterinary Medicine, Benha University

3 Animal Health Research Institute" Doki"


A total of 205 chicken samples from apparently healthy broiler chickens (35 and 30), diseased broiler
chickens (35 and 30) and freshly dead ones (35 and 40) were collected in winter (from December to
February) and summer (from June to August) seasons, respectively from Menofyiea government. The
results showed that the incidence of E.coli in apparently healthy broiler chickens was 15.7%, diseased
broiler chickens 37.1% and in freshly dead ones 55% in winter season while in summer season was
15.8% in apparently healthy, 17.5% in diseased broiler chickens and 18.7% in freshly dead one. The
serogroups of E.coli that obtained by serological identification were O128, O78, O111, O124, O55, O142, O114,O2 and O1. The results of antibiotic sensitivity test for isolated E.coli showed that the isolated E.coli
were highly sensitive for norfloxacin (60%), gentamycin(50%) , neomycin (50%) , streptomycin(50%)
and chloramphenicol (50%). moderately sensitive for doxcyclin (10%) and erythromycin(40%) and
highly resistant for amoxacillin /clavulinic acid (0%). The results of multiplex PCR showed that eae A(
intimin or E.coli attaching and effacing) gene detected in O128,O55, O1and O2, OmpA (outer membrane
protein ) gene detected in all E.coli serogroups that isolated , stx1 gene not detected in all E. coli
sergroups that isolated ,but stx2 gene detected in O114 and O128.