Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products

Ibrahim, Hemmat M.; Hassan, Mohamed A.; Amin, Reham A.; Eleiwa, Nesreen Z.; Nadim, Samaa S.

doi:10.21608/bvmj.2015.31735

Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products

Document Type : Original Article

Authors

¹ Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University

² Meat hygiene, animal health research institute Tanta Branch

10.21608/bvmj.2015.31735

Abstract

A total of 100 meat products samples of minced meat, kofta, beef burger and sausage (25 samples of
each), weight of each sample10gm were collected from different shops and supermarkets in Cairo
governorate, to be investigated for the presence of E. coli and detection of virulent genes by modern
technique (PCR). The obtained results indicated that the incidence of E. coli isolated from the examined
samples were (4)16%, (7)28%, (6)24% and (11)44% of minced meat, kofta, beef burger and sausage,
respectively. Actually E. coli strains isolated from 18 positive E.coli were 9 strains O₂₆, O₅₅:H₇, O₁₀₃,
O₁₁₁:H₄, O₁₁₄:H₂₁, O₁₁₉:H₆, O₁₂₄, O₁₂₅:H₂₁ and O₁₂₈:H₂. These isolated strains were investigated by using Multiplex PCR to detect presence of virulent genes (stx1, stx2 and eaeA) in each isolated strain. The
results obtained reported that E. coli O₂₆ & O₁₁₁ posses (3) genes stx1, stx2 and eaeA genes, E.coli O₁₀₃,
O₁₁₉ & O₁₂₈ carry (2) genes stx1and stx2, E. coli O₅₅ carry (2) genes stx1 and eaeA genes, E.coli O₁₂₅
carry (2) genes stx2 and eaeA genes, E. coli O₁₁₄ posses (1) gene stx1gene. While virulence genes were
not detected in E. coli O₁₂₄.

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