Biochemical markers to the protective effects of Fructus Piperis Longi extract on Hepatic encephalopathy in rats

AboZaid, Omayma A.R.; Mansour, Somaya Z.; El-Gendey, Abeer E.

doi:10.21608/bvmj.2015.31737

Biochemical markers to the protective effects of Fructus Piperis Longi extract on Hepatic encephalopathy in rats

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Benha University

² Department of Radiation Biology, National Centre for Radiation Research and Technology, Atomic Authority, Cairo, Egypt

10.21608/bvmj.2015.31737

Abstract

The present study was designed to elucidate the protective effect of subsequent pretreatment with
Fructus Piperis Longi extract in liver and brain tissue of experimental rats exposed to Hepatic
encephalopathy induced by intraperitoneal administration of TAA. Plf extract (50 mg/kg) was force-fed
to rats every day, 7 days before administration of Thioacetamide. Thioacetamide 200 mg/kg was
intraperitoneally administered to rats twice per week for four weeks. Administration of Plfext was
extended during Thioacetamide. After 4 weeks, animals were sacrificed. Biochemical parameters in
serum and homogenized liver and brain were tested. A significant increase in serum transglutaminase,
alkaline phosphatase and gamma glutamyl transferase activity and plasma ammonia, a significant
decrease in serum albumin and total protein was recorded in the liver of Thioacetamide-treated rats.
Significant increases in liver and brain lipid peroxides associated to significant decreases of liver and
brain reduced glutathione concentration and catalase activities were also recorded. Administration of
Plfext treatment reduced the severity of liver fibrosis and oxidative damage which was substantiated by
improvement of liver function detected by a decrease in serum aspartate amino transaminase, alanine
aminotransferase, alkaline phosphatase, gamma glutamyl transferase activities and increase in total
protein and Albumin. It was concluded that treatment of the ethanolic extract of Fructus Piperis Longi
enhance Liver and brain parameter and enhanced antioxidant activities of liver and brain.

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