Document Type : Original Article
Department of Avian and Rabbit Diseases, Faculty of Veterinary Medicine, Benha University, Egypt.
Laboratory For Veterinary Quality Control on Poultry production, Animal Health, Dokki, Giza, Egypt.
Several disease outbreaks were observed in duck farms in Egypt leading tonervous
manifestations and high mortality.In this investigation,suspected Duck Viral Hepatitis
samples (n=38) were collected from 38commercial flocks from five Egyptian governorates
including(Sharkia, Qalyubia,Manufia,Ismalia and Dakahlia) from December 2019 to
May2022 of age ranged from 3-16 days.Theinfected flocks were suffered from nervous
signs with high mortality ranged from (48%-91%).There is no history of previous vaccination
againstDuck Hepatitis A Virus(DHAV). Clinical samples were examined by Reverse
transcription-PCRdepend on molecular identification of UTR andVP1 generespectively. The
overall positiverate was 76.3% (n = 29/38). Out of 29 positive samples, five (17,24%) are
subjected to sequencing and phylogenetic analysis . Our findings demonstrated that these
novel DHAV strains were more similar and genetically correlated to the newly reported
DHAV genotype 3 in Egypt. There were amino acid substitutions detected in VP1 gene in
comparison to the first Egyptian genotype 3 (Avihepatovirus A isolate 26 VP).