Document Type : Original Article
Authors
1
Bacteriology, Immunology and Mycology Faculty of Veterinary Medicine - Menufia University, Sadat, Egypt
2
Bacteriology, Immunology, and Mycology Dep. Animal health Research Institute. Shebin El Kom, Menofia (AHRI).
3
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
4
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Abstract
Abstract
Forty-five random samples were collected from fresh broiler internal organs lungs, liver and kidney (15 samples each). All samples were screened for Staphylococcus aureus, Escherichia coli and Klebsiella. The bacteriological examination revealed the presence of E. coli (45/45 isolates), followed by Klebsiella (38/45 isolates) and S. aureus (16/45 isolates). The serotyping of E. coli (45) typed the strains as O111:H2 (60%), O128:H2 (8.8%), O113:H2 (6.6%), O114:H21 (6.6%), O26:H11 (6.6%) and O91:H12 (4.4%), O124 (2.2%), O103:H4 (2.2%), O55:H7 (2.2%). Biotyping of Klebsiella species (38) revealed that 19/38 (50%) strains were typed as k. pneumoniae, k. oxytoca 14/38 (36.9 %) and k. ozaenae 5/38 (13.1%). The antibacterial activity of Spirulina platensis water extract (10, 50, and 100 mg/dl) were tested in-vitro effect against Staphylococcus aureus, E. coli, and Klebsiella, using the paper disc diffusion method. Escherichia coli serotype O121 and S. aureus were the most susceptible strains to Spirulina platensis water extract with inhibition zones of (12,14,15mm) and (9,11,13mm) respectively, while minimal inhibitory effects were shown by Klebsiella, whose inhibition zone diameter was (0, 2, and 6 mm). Therefore, Spirulina Platensis water extract may be useful in various applications and be used as basic knowledge for further investigations.
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