Molecular studies on antimicrobial resistance genes in salmonella isolated from poultry flocks in Egypt

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University

2 Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Zagzig University

3 Animal Health Research Institute, Dokki Lab., Giza

4 Animal Health Research Institute, Dakahlia branch

Abstract

This study was aimed to investigate the wide spread resistance to different antimicrobial groups in
between different Salmonellae isolated from poultry flocks in Egypt. A total of 63 Salmonella isolates
(19 from chickens, 3 from ducks, 2 from turkeys, 2 from quails and 2 from pigeons) were examined in
this study for their wide spread resistance against different antimicrobial agents. The antimicrobial
susceptibility was applied on all isolates then PCR was applied for all resistant isolates to detect the
resistance genes of different antimicrobial agents (qepA, qnrS, aac (6') - ib-cr) for quinolone resistant
isolates, bla Tem for β lactam resistant isolates, sul1 for Sulfonamide resistant isolates, floR for
Florphenicol resistant isolates, aadA2 for Streptomycin resistant isolates and tetA (A) for
Oxytetracycline resistant isolates, in addition to gyrase enzyme gene (gyrA). DNA sequencing was done
for three selected isolates to detect possible mutations in the quinolone-resistance determining regions
of the gyrA gene

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