Antioxidant and anti-inflammatory effects of flaxseed oil and fish oil in fipronil induced oxidative stress in rats

Document Type : Original Article

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Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University

Abstract

The present study was designed to evaluate the antioxidant and inflammatory effect of flaxseed and fish oils in rats exposed to fipronil and bacterial wall containing lipopolysaccharides (LPSs). Sixty rats were divided into four groups each of 15 one. Group 1 served as control. Group 2, administered fipronil at dose of 15 mg/kg b.wt. orally for 15 days followed by single injection of LPSs (2 × 106 CFU/ rat) intraperitonially at day 16th. Group 3, treated as group 2 plus administration of fish oil at dose of 270 mg/kg b.wt. orally daily all over the experimental period (30 days). Group 4, treated as group 2 plus administration of flaxseed oil at dose of 270 mg/kg b.wt. orally daily all over the experimental period. Serum was separated and used directly for determination of urea, creatinine, albumin, sodium, potassium, Tumor necrosis factor –alfa and Interleukin 1β. Kidney samples were taken for histopathological examination, Malondiadehyde (MDA), Glutathione peroxidase (GPx) and Superoxide dismutase (SOD). Results revealed that in group 2 there were significant elevations of urea, creatinine, MDA, TNF-α and IL-1β. Furthermore, there were significant decreases in albumin, sodium, potassium, GPx and SOD compared with control group. Meanwhile, administration of flaxseed and fish oils resulted in significant decrease in urea, creatinine, MDA, TNF-α and IL-1β and significant increase in albumin, sodium, potassium, GPx and SOD compared with group 2. Therefore it's concluded that flaxseed and fish oils have strong antioxidant and anti-inflammatory effects.

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Benha Veterinary Medical Journal
Volume 35, Issue 2
December 2018
Pages 44-56

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